FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005086634

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005086634
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17557667
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCACTCA60100.018.9252839
GCCACTC91350.012.6087258
AGCCACT107050.010.9612977
ACACTAT62550.09.6674458
AAGCCAC124750.09.52123456
AACACTA64800.09.4428897
TATGTTA78900.07.84652763
TACAAAG178000.07.28131772
CTAGCTC20850.07.251740545-49
CACTATA89850.07.1306929
ACAAAGC176700.07.00725563
GTTATGT93250.06.9508141
ATGTTAA96650.06.9267144
TGTTAAT100000.06.91060645
GTTAATT102650.06.87253576
CTCACTA36450.06.5965815-19
ATACAAA216100.06.27140571
AAACACT101250.06.18548976
CAAACAC101200.06.04621125
AAAGCCA219600.05.90044935
Produced by FastQC (version 0.11.5)