FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005086800

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005086800
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15834803
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTTAA31800.08.3793154
AGAGCGT194000.06.27204568
GATCGGA201650.06.037841
TACGTTA45000.05.92138343
CTAGACC20700.05.634871120-124
ACCATAC118400.05.5332249
ATACAGC125300.05.46217541
TACAGCC124650.05.43087152
GAGCGTC252550.05.4162019
GTTACGT64800.05.3365351
AGCCACC122200.05.30293275
CACCATA126900.05.27649558
TCGGAAG250350.05.17796233
AAGAGCG234250.05.00992067
Produced by FastQC (version 0.11.5)