FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005086860

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005086860
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17940342
Sequences flagged as poor quality0
Sequence length150
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACTAT49100.014.8095418
CACTATA59600.013.7704919
AACACTA56200.013.7072137
AAACACT82350.09.1794226
CAAACAC92300.08.2678735
GCCCTTA58550.07.99237974
CCAAACA103800.07.62930444
ACCAAAC108800.07.27869373
TAGCCCT80050.05.75601052
CTCACTA24209.4114275E-65.6524927
TACCAAA160550.05.42597772
ACTATAG36150.05.3373910-14
TATTTAG86900.05.30213749
CTATAGC38400.05.13714510-14
Produced by FastQC (version 0.11.5)