FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005086909

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005086909
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16102133
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA127250.017.1975259
AGAGCAC132300.016.2690248
GATCGGA141800.015.2524911
TCGGAAG226100.09.9425583
ATCGGAA234250.09.7828312
AAGAGCA240150.09.5022577
AGTGAGT44200.08.07298851
GAAGAGC291700.07.9957416
TAACCCC43550.07.76890569
GTGAGTA41750.07.76735262
GGAAGAG314000.07.29036055
AGTCACA60900.07.116201425-29
CTAGATC9250.06.226481140-144
CGGAAGA409700.05.74566174
CAGTCAC79900.05.478048325-29
CACACGT91450.05.179553510-14
GTCACAG88800.05.172217425-29
CACGTCT93800.05.04978910-14
Produced by FastQC (version 0.11.5)