FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005087054

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005087054
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13629800
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGAGT30250.016.8472711
GTGAGTA31700.015.6723752
TAACCCC37650.013.5763379
GAGTATA69250.08.2138044
ATAACCC60750.07.9399498
AGAGCGT217250.07.48924168
TCGGAAG219150.07.45803743
GATCGGA225550.07.39178851
TGAGTAT72200.07.3796323
GTATAAC70150.07.28651576
GAGCGTC252550.06.64198269
ATCGGAA256300.06.4613642
AAGAGCG260700.06.3514967
TATAACC80600.06.25247867
CTAGATC13000.06.2026544140-144
TAGATCT58500.05.95611140-44
GAAGAGC299300.05.67668156
CTAACTA19500.05.538491125-129
CGGAAGA319100.05.3927234
GGAAGAG319250.05.34425935
Produced by FastQC (version 0.11.5)