FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005087131

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005087131
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences86669944
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA530350.023.293821
ATCGGAA560350.022.1782
TCGGAAG569300.021.7619923
CGGAAGA605150.020.49634
TATGCCG206500.013.27036745-49
AGAGCAC1061000.011.9769858
CTCGTAT232000.011.53079940-44
TCTCGTA222650.011.47451340-44
CGTATGC238650.011.43403145-49
GAGCACA1140050.011.1846649
GAAGAGC1184150.010.8415856
ATGCCGT258050.010.31034945-49
GCCGTCT263450.010.30907350-54
TGCCGTC255400.09.92431650-54
TCGTATG260900.09.57529945-49
ATCTCGT270250.09.37835840-44
CGTCTTC312400.08.93510750-54
CCGTCTT315150.08.82953350-54
CATCTCG313550.08.26821540-44
AAGAGCA1647150.08.1593887
Produced by FastQC (version 0.11.5)