FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005087153

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005087153
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences133938344
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGATC103600.016.165293
GATCGGA821550.014.7505081
ATCGGAA890550.013.6803912
TCGGAAG912650.013.4567583
CGGAAGA948250.012.9050154
CCGATCT151550.011.6240974
TATGCCG298150.011.54963345-49
TTCCGAT168450.010.7625832
CGTATGC339700.09.94488645-49
CTCGTAT344000.09.81216440-44
TCTCGTA331850.09.69954640-44
ATGCCGT380400.08.97995645-49
GCCGTCT400250.08.50208950-54
CTTCCGA218350.08.2665681
ATCTCGT405100.08.03157840-44
AGAGCAC1685450.07.66914468
CGTCTTC472850.07.59839450-54
GAGCACA1799250.07.2727259
CCGTCTT487250.07.20719450-54
GAAGAGC1882650.06.9698036
Produced by FastQC (version 0.11.5)