FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005087187

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005087187
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70619084
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAAGGTGAATCTCGTAT1975410.27972750255440865TruSeq Adapter, Index 2 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA832200.041.514691
ATCGGAA887100.038.910322
TCGGAAG893600.038.48443
CGGAAGA924650.037.035294
AGAGCAC1256350.027.297248
GAGCACA1309700.026.3070499
GAAGAGC1377100.024.982686
TATGCCG417400.020.36028545-49
AAGAGCA1723150.020.209547
TCTCGTA421700.018.95996540-44
CGTATGC444050.018.6060945-49
CTCGTAT443750.018.56353840-44
GGAAGAG1923400.018.18845
ATGCCGT455700.018.03178645-49
GCCGTCT461500.017.644150-54
ATCTCGT464600.016.934640-44
CGTCTTC501350.016.73618950-54
CCGTCTT501900.016.41451850-54
TGCCGTC456750.014.86584950-54
TCGTATG474000.014.49376945-49
Produced by FastQC (version 0.11.5)