FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005087219

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005087219
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences128517370
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA793300.020.0086481
ATCGGAA852500.018.6859552
TCGGAAG876600.018.2032073
CGGAAGA924800.017.1760754
TATGCCG311300.013.00401745-49
TCCGATC96400.011.7321313
CGTATGC348700.011.52610445-49
CTCGTAT350600.011.30642340-44
TCTCGTA338300.011.2374940-44
ATGCCGT383800.010.39266445-49
AGAGCAC1623900.010.2361748
GCCGTCT400800.09.93727450-54
GAGCACA1741900.09.6551199
GAAGAGC1821650.09.2762026
ATCTCGT415250.09.24236140-44
CGTCTTC482100.08.67350150-54
TCGTATG379950.08.55559845-49
CCGATCT143300.08.5500744
CCGTCTT491250.08.32305750-54
TGCCGTC393350.08.28984950-54
Produced by FastQC (version 0.11.5)