FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005087247

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005087247
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences81820374
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCGAC45500.011.8573851
GATCGGA584800.011.7438941
ATCGGAA602450.011.5892742
TCGGAAG610300.011.462773
CGGAAGA631850.011.0259264
TATGCCG158100.010.06962645-49
CGTATGC178700.08.51125945-49
CTCGTAT184750.08.45216640-44
TCTCGTA188750.08.18086740-44
GCGACCA72300.08.0215323
ATGCCGT206900.07.75764745-49
GCCGTCT214500.07.29356750-54
GGCGACC79300.07.2302962
TCGTATG204700.06.9768845-49
CGACCAC85650.06.94052174
TGCCGTC213400.06.937088550-54
AGAGCAC1059100.06.91336548
ATCTCGT234600.06.569646440-44
GAGCACA1141750.06.51450549
CGTCTTC262300.06.434289550-54
Produced by FastQC (version 0.11.5)