FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005087258

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005087258
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65217026
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGTC369650.024.4168829
AGAGCGT409500.022.146638
GATCGGA415550.021.8838731
ATCGGAA447100.020.5296672
TCGGAAG461000.019.8947353
AAGAGCG477800.019.1942237
CGGAAGA482450.019.0845224
CGCCGTA114200.018.5744955-59
TCGCCGT129300.016.37167255-59
GTCGCCG145050.014.92384655-59
GCCGTAT147900.014.56764355-59
TGGTCGC167100.013.10232450-54
CGTATCA173550.012.390305560-64
GTGGTCG181500.012.38234550-54
CCGTATC168300.010.72631960-64
GGTCGCC173350.010.33792455-59
GAAGAGC940550.010.2822496
TCTCGGT230100.010.08787945-49
CTCGGTG263100.08.88319945-49
CGGTGGT258800.08.72312650-54
Produced by FastQC (version 0.11.5)