FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005087290

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005087290
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15554464
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCACTCA58650.023.18999
GCCACTC71950.019.1032668
ACGTTAA41800.017.0447484
AAGCCAC105650.013.214226
AGCCACT106900.012.6556747
TACGTTA63750.011.2890773
ACAAAGC143550.010.3778113
CGTTAAA68950.010.1238755
ATTACGT75850.010.0121031
TACAAAG171850.08.585882
CTCACTA35500.08.55849315-19
ATACAAA196300.07.88611561
GTTAAAT102750.07.14377266
TTACGTT110050.07.12880852
AAAGCCA217500.06.8488935
CCTAATG46250.06.6623526105-109
CCCCTAA48900.06.359786100-104
CCCTAAT48750.06.349821100-104
TACACAC49600.06.327639650-54
ACTCACT49500.06.312446615-19
Produced by FastQC (version 0.11.5)