FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005087309

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005087309
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences83259790
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA465650.012.4859791
ATCGGAA496450.011.8335692
TCGGAAG510000.011.50163
CGGAAGA533750.011.1244024
TATGCCG148050.010.11680745-49
CGTATGC172150.08.71735845-49
CTCGTAT171450.08.71051740-44
TCTCGTA171050.08.47658940-44
ATGCCGT195850.07.714285445-49
GCCGTCT204550.07.372100450-54
ATCTCGT218750.06.734259640-44
TCGTATG193150.06.500918445-49
AGAGCAC990400.06.2949298
CGTCTTC256750.06.200822450-54
TGCCGTC198250.06.18749150-54
GAGCACA1066850.05.9933419
CCGTCTT265050.05.951939650-54
CATCTCG260550.05.754050340-44
GAAGAGC1116900.05.7052886
AGATCGG466450.05.5343623145
Produced by FastQC (version 0.11.5)