FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005087344

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005087344
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46559473
Sequences flagged as poor quality0
Sequence length151
%GC44

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG72950.010.137059145
CGCCGTA126450.06.3865555-59
GCCGTAT139400.05.99086755-59
CGTGTAG557250.05.581415145
TCGTGTA549300.05.4510174145
CGTATCA158850.05.412303460-64
AGCGTCG642400.05.338174145
TCGCCGT154550.05.32855355-59
CGTCGTG583000.05.2975874145
GCGTCGT595700.05.111649140-144
AGATCGG926800.05.014286145
Produced by FastQC (version 0.11.5)