FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005087422

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005087422
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73874937
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGTC404500.012.3139149
AGAGCGT460250.010.869548
CGCCGTA108250.010.54079455-59
GATCGGA487350.010.2303831
ATCGGAA504350.09.90720752
TCGGAAG522550.09.6009613
TCGCCGT125300.09.21061255-59
AAGAGCG556600.09.11819657
CGGAAGA560650.09.0519064
GCCGTAT141350.08.23656755-59
GTCGCCG145100.08.11362855-59
TGGTCGC167350.07.112570350-54
CGTATCA176550.06.8239760-64
GTGGTCG180800.06.67968150-54
GGTCGCC174300.06.11387355-59
CCGTATC169650.06.04184260-64
TAGATCG186500.05.7914248145
AGCGTCG333450.05.500065145
CGTCGTG304550.05.212709145
GAAGAGC1071150.05.1916186
Produced by FastQC (version 0.11.5)