FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005087432

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005087432
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18692663
Sequences flagged as poor quality0
Sequence length150
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA199600.010.6211871
TCGGAAG211350.09.399813
CCACTCA48100.08.9799079
AGAGCGT232000.08.7814078
ATCGGAA252950.08.2256142
AAGAGCG278200.07.8924097
TAGTGTA55800.07.09555735-39
AGTGTAG58550.07.081961635-39
GAGCGTC330200.06.4532779
GAAGAGC335050.06.40302376
ATAGTGT63650.06.35639730-34
GGAAGAG330150.06.3236115
GCCACTC81500.06.0064388
TAGATCT72350.05.91048840-44
CGGAAGA367400.05.7216614
CTCACTA19250.05.60896815-19
AGCCACT91450.05.5103757
Produced by FastQC (version 0.11.5)