FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005087436

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005087436
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71734986
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA115650.012.70069155-59
TCGCCGT134150.010.97081755-59
GCCGTAT150900.09.88757355-59
GTCGCCG153250.09.56564555-59
GAGCGTC489700.08.8370879
CGTATCA181150.08.37990560-64
TGGTCGC179050.08.33222750-54
AGAGCGT544150.08.2192768
GTGGTCG194200.07.83152450-54
GATCGGA579150.07.615761
ATCGGAA608850.07.2394322
AGCGTCG408400.07.09968669
TCGGAAG625650.07.04470973
AAGAGCG640000.06.98832137
CGGAAGA655800.06.7871574
TCTCGGT258250.06.220475745-49
CGTCGTG381000.05.4804893145
AGATCGG601300.05.474147145
CCGTATC174650.05.42925160-64
CGGTGGT294550.05.41444250-54