FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005087449

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005087449
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences69595707
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAAGGTGAATCTCGTAT1139780.16377159585432474TruSeq Adapter, Index 2 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA556150.033.874471
ATCGGAA589950.032.1431852
TCGGAAG597900.031.4005073
CGGAAGA621850.030.0743374
AGAGCAC974550.019.2867818
TATGCCG236150.018.6235645-49
GAGCACA1035400.018.2093129
GAAGAGC1081300.017.7715346
CGTATGC262350.016.26072345-49
CTCGTAT263850.016.19273640-44
TCTCGTA264450.016.07376540-44
ATGCCGT278900.015.30620645-49
GCCGTCT283850.014.96617850-54
ATCTCGT302850.013.75324640-44
CGTCTTC326650.013.51120850-54
AAGAGCA1445500.013.499527
TGCCGTC279600.013.40465350-54
TCGTATG285300.013.22971945-49
CCGTCTT332450.012.97020450-54
GGAAGAG1641150.012.0624295
Produced by FastQC (version 0.11.5)