FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005087482

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005087482
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences69942504
Sequences flagged as poor quality0
Sequence length151
%GC41

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGTC407600.010.879649
GATCGGA465550.010.0107031
AGAGCGT456450.09.8424228
ATCGGAA492700.09.5072392
CGCCGTA137650.09.45436755-59
TCGGAAG509300.09.0788973
CGGAAGA542850.08.5569624
TCGCCGT155000.08.42413755-59
AAGAGCG547150.08.3699627
GCCGTAT167150.07.97652655-59
GTCGCCG185300.06.937140555-59
TGGTCGC202850.06.615588750-54
CGTATCA200900.06.600399560-64
GTGGTCG219150.06.24256450-54
CGGTGTA220350.05.971918635-39
GCGTCGG65150.05.454502145
ACCGGTG245200.05.449435735-39
AGCGTCG341200.05.33042679
CACCGGT247750.05.247105635-39
TCTCGGT283050.05.027837845-49
Produced by FastQC (version 0.11.5)