FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005087483

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005087483
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68344212
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA397850.011.7387661
ATCGGAA431150.010.9181022
TCGGAAG443400.010.6114073
CGGAAGA466600.010.1302134
TATGCCG145100.010.092689545-49
CTCGTAT163050.09.14205240-44
CGTATGC161800.08.91656345-49
TCTCGTA157700.08.78098240-44
ATGCCGT185350.08.0026945-49
GCCGTCT194700.07.498975850-54
ATCTCGT199250.07.109967740-44
CGTCTTC233700.06.70044250-54
CCGTCTT235300.06.445374550-54
AGAGCAC839700.06.08629138
GAGCACA909300.05.6443489
AGATCGG398600.05.5678434145
TCGTATG182500.05.55350645-49
GAAGAGC945700.05.48848446
TGCCGTC187200.05.41389450-54
AATCTCG301250.05.053981340-44
Produced by FastQC (version 0.11.5)