FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005087539

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005087539
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences75222318
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTCTCATCTCGTAT954910.12694503777456045TruSeq Adapter, Index 5 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA638050.025.7801231
ATCGGAA655600.025.1230622
TCGGAAG660650.024.809113
CGGAAGA682300.023.8944054
TATGCCG211450.017.15114245-49
AGAGCAC1047500.015.7505198
CGTATGC234800.014.98851845-49
GAGCACA1112000.014.849979
CTCGTAT239300.014.80840540-44
TCTCGTA240950.014.73106840-44
GAAGAGC1167600.014.2607896
ATGCCGT256650.013.88196345-49
GCCGTCT261200.013.38926650-54
TCGTATG259450.012.82673345-49
TGCCGTC258350.012.80175450-54
ATCTCGT282800.012.30499940-44
CGTCTTC312450.011.75458450-54
CCGTCTT317500.011.21141450-54
CATCTCG310600.011.17097340-44
AAGAGCA1564100.010.8495957
Produced by FastQC (version 0.11.5)