FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005087625

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005087625
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16517644
Sequences flagged as poor quality0
Sequence length150
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGAGT19450.011.9734521
GTGAGTA23000.010.330972
TAACCCC36650.07.65950979
TGAGTAT54550.05.41110853
TATAACC63650.05.20199357
ATAACCC55203.6379788E-125.08552658
GTATAAC55253.6379788E-125.08089356
GAGTATA56701.8189894E-125.0779984
Produced by FastQC (version 0.11.5)