FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005087627

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005087627
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61287842
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA336250.012.7417111
ATCGGAA367400.011.7638452
TCGGAAG383300.011.1217193
TATGCCG130800.010.81956245-49
CGGAAGA406600.010.5733844
CTCGTAT149750.09.27570640-44
TCTCGTA146650.09.20483340-44
CGTATGC149900.08.99598945-49
ATGCCGT171400.08.3074645-49
CTTACGG177750.07.929061430-34
GCCGTCT179700.07.738348550-54
TTACGGT180250.07.682334430-34
ATCTCGT177200.07.421503540-44
CGGTGAT184950.07.039942735-39
ACTTACG197150.07.03114830-34
CGTCTTC215050.06.80345450-54
CCGTCTT217000.06.475029550-54
AGAGCAC733750.06.25407558
GAGCACA798400.05.8202969
GAAGAGC823500.05.81015736
Produced by FastQC (version 0.11.5)