FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005087646

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005087646
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59788176
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGTC412600.016.1386689
AGAGCGT452350.014.8005828
CGCCGTA110800.014.36265855-59
GATCGGA475300.014.23411751
ATCGGAA500850.013.5014812
TCGGAAG515400.013.0741913
TCGCCGT123550.012.89220455-59
AAGAGCG526900.012.7890517
CGGAAGA540250.012.5534734
GTCGCCG138450.011.45240255-59
GCCGTAT141350.011.35073755-59
TGGTCGC155450.010.32125450-54
CGTATCA168950.09.61702560-64
GTGGTCG168800.09.55648750-54
CCGTATC159900.08.47532860-64
GGTCGCC163400.08.17807655-59
GAAGAGC948750.07.6600766
TCTCGGT222200.07.61848345-49
CGGTGGT242850.06.95882650-54
CTCGGTG245600.06.916222645-49
Produced by FastQC (version 0.11.5)