FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005087661

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005087661
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71052319
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGGAGCTGATCTCGTAT715350.10067933180337155TruSeq Adapter, Index 10 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA657050.018.360681
ATCGGAA680150.017.8728962
TCGGAAG686450.017.6890543
CGGAAGA711100.017.0452884
TATGCCG206300.013.9517445-49
CTCGTAT231650.012.2993240-44
TCTCGTA221300.012.26521940-44
CGTATGC230750.012.15295545-49
AGAGCAC1052450.011.8680458
ATGCCGT246200.011.47865345-49
GAGCACA1118650.011.2175569
GCCGTCT250250.011.04852350-54
GAAGAGC1194800.010.5995986
ATCTCGT262950.010.01367640-44
CGTCTTC294750.09.96582450-54
TGCCGTC248350.09.88371950-54
TCGTATG252550.09.8637745-49
CCGTCTT300450.09.34244950-54
AAGAGCA1541250.08.4569167
GGAAGAG1746600.07.5165185
Produced by FastQC (version 0.11.5)