FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005087673

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005087673
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences129991440
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCGAC149750.017.9716191
GCGACCA175650.016.1753433
GGCGACC215350.013.6036632
CGACCAC223050.012.9978944
ACCGAGA295400.09.8133429
CCACCGA329550.09.2803737
CACCGAG405250.07.54681258
ACCACCG407500.07.4340066
TATGCCG433150.06.150427345-49
CGACGCT98400.05.93619345-49
ACGACGC93750.05.921416345-49
ATGCCGT506300.05.40781945-49
CTCGTAT503050.05.32750540-44
CGTATGC490750.05.28086745-49
GATCGGA2141300.05.268961145
CGTCTGA1356100.05.267923145
TCTCGTA493300.05.25063240-44
ACACGTC1500100.05.210676145
ATCGGAA2136650.05.1332736145
AGATCGG2196750.05.0427628145
Produced by FastQC (version 0.11.5)