FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005087720

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005087720
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49118299
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTACAGGTCGTGTAGATCT635860.1294548086854555Illumina Single End PCR Primer 1 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA359550.031.4978831
GAGCGTC329450.031.0076229
ATCGGAA380000.029.4961812
AGAGCGT358400.028.7861718
TCGGAAG393700.028.2098063
CGGAAGA407200.026.6692774
AAGAGCG411750.025.4239487
CGCCGTA138600.019.28200355-59
TCGCCGT152200.017.45423955-59
GCCGTAT162750.016.5811855-59
GTCGCCG166450.015.62019955-59
TGGTCGC180650.015.15400350-54
GAAGAGC743900.014.8617066
GTGGTCG189850.014.38910150-54
CGTATCA183750.014.172379560-64
AGGTCGT204050.012.69111335-39
GGTCGTG219100.012.355435-39
TCTCGGT229650.012.324326545-49
CTCGGTG249100.011.39113445-49
CCGTATC175700.011.32259960-64
Produced by FastQC (version 0.11.5)