FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005087745

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005087745
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17028143
Sequences flagged as poor quality0
Sequence length150
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA134900.019.9431931
GAGCACA163750.016.1643629
ATCGGAA199000.013.7386012
AGAGCAC193450.013.7196598
TCGGAAG231950.011.8797883
AAGAGCA248950.011.007757
GAAGAGC325000.08.6748936
GGAAGAG385150.07.30143645
CACACGT86100.06.532675310-14
ACTAATC40550.06.491835635-39
CGGAAGA432100.06.4748074
CACTAAT40950.06.28791135-39
GTCACTA44250.05.981523535-39
AGTCACA54150.05.882029525-29
CTAATCT42650.05.666273635-39
CCAGTCA79150.05.63752625-29
TCACTAA47800.05.53728935-39
ACTCCAG100700.05.34278320-24
AGTCACT51350.05.337315630-34
Produced by FastQC (version 0.11.5)