FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005087763

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005087763
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17401144
Sequences flagged as poor quality0
Sequence length150
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCCTTTGACATATTCCACAACCCTATGAATTACCTCTACTGTGTGTGCA532180.305830467238246No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACATAT162700.049.5588658
TGACATA163650.049.183337
ACATATT165200.048.9832049
TTTGACA166250.048.890495
TTGACAT169750.047.7976236
AGCCTTT231750.035.405291
CCTTTGA261300.031.3424453
CTTTGAC269600.030.7895134
GCCTTTG360000.023.109342
TACTAAG9750.018.4690062
CTAAGAG9400.018.3813234
ACTAAGA10350.018.0944273
TACTGTG128450.013.36219435-39
GGACCCA82000.013.3451017
ACTGTGT127650.013.32185535-39
CAACCCT134500.012.85727315-19
CTACTGT132500.012.83422535-39
ACCCTAT134850.012.75989320-24
CCCTATG134050.012.69640520-24
CTAGCTA2950.012.69066825-29
Produced by FastQC (version 0.11.5)