FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005087825

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005087825
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64326469
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG118700.010.54252245-49
GATCGGA501500.010.0376071
TCGGAAG519050.09.9579763
ATCGGAA517600.09.8743122
CGGAAGA534150.09.5407574
CTCGTAT142250.08.93918440-44
TCTCGTA141350.08.74991540-44
CGTATGC137000.08.64740845-49
ATGCCGT161000.07.889739545-49
GCCGTCT161250.07.346499450-54
TCGTATG156000.06.757627545-49
ATCTCGT182450.06.58016940-44
CGTCTTC202300.06.579682450-54
TGCCGTC158750.06.557960550-54
AGAGCAC862550.06.2443648
AGATCGG507350.06.2312512145
GAGCACA907350.06.01594459
CCGTCTT209500.06.000579450-54
GAAGAGC975200.05.61968236
TAGATCG189850.05.3852386145
Produced by FastQC (version 0.11.5)