FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005087858

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005087858
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences83720222
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA138100.012.43053955-59
GAGCGTC588650.011.8240769
TCGCCGT159100.010.90827155-59
AGAGCGT658400.010.7254498
GATCGGA691450.010.1358251
GCCGTAT177800.09.93224655-59
ATCGGAA715800.09.7850762
TCGGAAG734850.09.579833
GTCGCCG183700.09.35278955-59
AAGAGCG776900.09.1921587
CGGAAGA775400.09.1816864
CGTATCA213500.08.39350860-64
TGGTCGC211050.08.32656550-54
GTGGTCG233650.07.707337450-54
CCGTATC205050.06.964651660-64
GGTCGCC216900.06.55086355-59
TCTCGGT303350.06.160997445-49
GAAGAGC1307250.05.8344736
CTCGGTG347750.05.51196545-49
CGGTGGT345300.05.45037950-54
Produced by FastQC (version 0.11.5)