FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005087863

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005087863
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64969546
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA328850.015.4116031
ATCGGAA354050.014.5413052
TCGGAAG370800.013.9595623
CGGAAGA391300.013.1355934
TATGCCG123950.011.334784545-49
CTCGTAT138950.010.05924440-44
TCTCGTA138050.09.47364240-44
CGTATGC141350.09.09837545-49
ATGCCGT169150.08.59735745-49
GCCGTCT176200.07.463421350-54
ATCTCGT178100.07.21301240-44
AGAGCAC765950.07.164748
CGTCTTC211850.06.93294250-54
TCGTATG158350.06.87634945-49
GAGCACA822600.06.78589259
CCGTCTT213400.06.6040250-54
GAAGAGC844450.06.55023536
TGCCGTC171050.06.476000350-54
AGATCGG326250.05.22507145
Produced by FastQC (version 0.11.5)