FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005087878

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005087878
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15920030
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACACTA62750.029.714597
ACACTAT66350.028.1026958
CACTATA75450.024.8087359
CAAACAC101500.019.1496565
AAACACT104750.018.5563876
TCAAACA138550.013.8229184
GCCCTTA79500.013.6752244
TAGCCCT83350.012.5253862
ATCAAAC164150.011.9304473
TATTTAG94250.011.3813939
GTAGCCC121500.08.8670581
CCCTTAT128850.08.4363735
CCTTATT139100.08.2808816
AGCCCTT139800.07.93126153
TATCAAA278100.07.6374742
ATATCAA273050.07.6246161
ACTATAG58050.07.217770610-14
CTATAGC58200.07.1249510-14
TACACTC57850.06.9453163115-119
CTTATTT189150.06.54644877
Produced by FastQC (version 0.11.5)