FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005087960

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005087960
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12929241
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCTAA12550.021.238123
CGACTAA17700.014.2446843
GAGCTAA26300.013.4214173
TAGGCTA23050.012.1927412
CGTCTAA19450.011.481543
AGATCTA28550.010.6010992
ACTCTAA23900.010.5494113
CTACTAA25550.09.8681373
TAGCTAA24500.09.7029973
GACTCTA24700.09.3359972
TACGTAA38000.09.0994623
GCTCTAA23600.08.8520543
TTAGGCT28900.08.6444841
TATACTA42400.08.1579882
GTACGTA42100.07.87378262
CTCAGAT58250.07.568561
CTATACT43200.07.4838811
TGAGCTA45250.07.32566262
ATACTAA40900.07.22135353
CTCCTAA42750.07.0773593
Produced by FastQC (version 0.11.5)