FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005087967

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005087967
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10451873
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCCAA48250.024.7717361
CCCCAAA70150.018.0636372
GGCCTAG4109.657768E-615.804432
GCCTAGT4903.1943346E-614.6937433
CCCAAAA88150.014.0486523
CCTAGTG5851.3410845E-613.5368484
CCAAAAA124850.010.5522074
CTAGTGC6557.695898E-49.8919515
CCCCCTA18100.09.6636811
GAGCACA79200.08.0898679
AGAGCAC88550.07.2357278
GGTATAC37550.07.1812771
GGTGTGA54000.07.01809841
GGTGTAG95700.06.93009761
GGTGTTT81200.06.73155071
GGTGTAA54850.06.64359661
GGTGTAC67050.06.30433231
CAAAAAA228650.06.1711425
GGTGTTG128850.06.1087061
GGTAATG68600.06.0556481
Produced by FastQC (version 0.11.5)