FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005088006

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005088006
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10205390
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACTAA13250.028.8182013
CGTCTAA21650.022.2958353
GATCTAA26550.020.3518373
GAGCTAA31500.018.297273
GTACGTA35000.014.0026982
ACAGTGT33750.013.9321061
CGTACGT34300.013.9229031
CAGTGTA40100.012.4015392
CACGTAA37950.012.3398083
TGAGCTA47750.011.6221962
AGATCTA49000.011.6198852
CTCCTAA42050.011.4793063
AGTGTAA42700.011.13583853
TAGCTAA41650.011.0706183
GACTCTA38450.011.0592732
GTAGCTA42750.010.9584252
TAGGCTA39150.010.8615322
TAGAGTA51400.010.79688552
ATACTAA58450.010.7236233
TCACGTA42950.010.5717832
Produced by FastQC (version 0.11.5)