FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005088042

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005088042
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15022757
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGAGT53500.033.1435741
GTGAGTA56350.031.5631392
TAACCCC73200.023.0130149
ATAACCC101400.017.1098568
GTCTCTT66200.013.6139741
GAGTATA161500.011.503354
TGAGTAT167000.011.0813793
GTATAAC183950.010.2536266
TATAACC207550.08.7755387
CGCCTAA53000.07.090369745-49
AGCATGA169250.06.8480349
CTCTTCA161500.06.6879943
ACTACCC57950.06.63378135-39
CAGCATG177800.06.2757738
CTACCCC63150.06.11033235-39
GACCCTA45500.05.854116495-99
AACCCCC66800.05.6255110-14
CCGCCTA74950.05.57095940-44
TCTCTTC187350.05.49616152
GCCTAAA74150.05.436896345-49
Produced by FastQC (version 0.11.5)