FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005088073

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005088073
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22055439
Sequences flagged as poor quality0
Sequence length150
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCTAG13800.052.694663
ATCTAGC14650.050.131254
TCTAGCC16900.042.597345
CTAGCCG18800.038.6761746
TGATCTA63900.011.4952182
GAGCACA219650.010.6518799
GATCGGA220650.010.6482111
TCGGAAG279700.010.55396653
AGAGCAC238900.09.9745168
ATCGGAA311200.08.0298562
AAGAGCA335950.07.5430687
CGGAAGA455650.06.7632964
CCTAATG33654.3655746E-116.36746261
GAAGAGC422250.06.15485146
GGAAGAG422550.05.87773755
AACACTA51750.05.84277157
TAGCCGG153100.05.5956667
CACTATA55150.05.221429
CTCGTAT102850.05.01129940-44
Produced by FastQC (version 0.11.5)