FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005088114

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005088114
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20173673
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGATACTAGTCGTGTAGATCTCGGT351260.17411802005514812Illumina Single End PCR Primer 1 (96% over 28bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA328050.019.1686081
TCGGAAG387250.016.3036983
GAGCGTC385800.015.6743539
AGAGCGT411200.014.86370858
ATCGGAA435350.014.7670262
AAGAGCG471900.013.394217
CTAGTCG88750.013.35165430-34
ACTAGTC89700.013.11394230-34
CGGAAGA515450.012.4442614
TACTAGT101150.011.64374325-29
ATACTAG111350.011.04263825-29
GAAGAGC591350.010.9321176
GGAAGAG599350.010.8824245
TAGATCT123300.010.36942440-44
AGTCGTG143000.08.9207530-34
AGATCTC159000.08.39435540-44
GTAGATC158500.08.37539135-39
TAGTCGT158200.08.11824330-34
AACACTA74200.08.0529372
ACACTAT76200.07.8415353
Produced by FastQC (version 0.11.5)