FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005088217

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005088217
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14896493
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACTAT41950.011.8401028
CACTATA41450.011.6356359
AACACTA50400.09.8550067
CAAACAC74500.06.6671235
AAACACT77200.06.52719166
CCAAACA92500.06.07009654
ACCAAAC97450.05.7617643
TAGCCCT70350.05.3288572
CACACGT128500.05.1874204140-144
GCCCTTA60100.05.03057434
Produced by FastQC (version 0.11.5)