FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005088310

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005088310
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13847018
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCA32200.045.8341757
TAGACTC26500.045.6406365
ACTCACA43650.035.1318369
TACTCAC43950.034.072978
GTAGACT40500.031.1099644
AGAGTAG40000.030.9769861
TCTACTC55550.026.6979376
AGTAGAC48150.025.5692443
GAGTAGA52550.024.387922
ACTCTCA51700.024.2306028
GACTCTC53800.024.2207577
CTCTACT66600.022.9165845
AGACTCT58350.022.0854746
CTCTCAA68100.019.2410959
TCCTCTA85600.017.9152953
CCTCTAC125850.012.528744
ATCCTCT128950.012.1720192
TATCCTC147500.011.4823931
TAGCATG35800.010.2553935-39
AGGGATC51950.07.64902490-94
Produced by FastQC (version 0.11.5)