FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005088329

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005088329
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14918155
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGAGCCCACAAGGCCCTAAAATATACACAATAAGGCAATCCCAGAGCCC252460.1692300421868522No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAGCCC76900.051.661212
GAGCCCA80700.049.847643
AGCCCAC101400.039.3985754
CCCACAA112500.035.6392176
GCCCACA125400.032.0303885
CACAAGG131100.030.8573868
ATGAGCC135100.029.9335541
CCACAAG137750.029.5767427
ACAAGGC186550.022.0712179
GGGCCTA52400.015.79851465-69
CCTATAG55000.014.81627170-74
TAGGGCC56850.014.38459165-69
TTAGGAC56650.013.3443985135-139
GAGCACA162050.012.5263819
CTATAGT67500.012.456449570-74
AGAGCAC166400.012.2854348
AGCCCTA72250.011.79639245-49
TAGGACC58750.011.568413135-139
GTTTAGC73350.011.38558375-79
GAGCCCT74500.011.20823245-49
Produced by FastQC (version 0.11.5)