FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005088348

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005088348
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16525315
Sequences flagged as poor quality0
Sequence length150
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACACTA44950.016.1779777
ACACTAT49250.015.0578688
CACTATA48150.014.8037399
AAACACT82550.09.1580866
CAAACAC112300.07.18076565
GATCGGA159200.06.9648241
GCCCTTA56950.06.4477614
ATCAAAC134900.06.0845073
TAGCGTA37600.06.05106430-34
TATTTAG62500.05.99039949
TCAAACA139400.05.9397424
TCGGAAG180250.05.91178943
TAGCGTG38200.05.842931735-39
ATCGGAA197200.05.84178542
AGAGCGT199850.05.4040538
CACCCTA35150.05.366714575-79
AAGAGCG226250.05.1553597
TAGCCCT81400.05.13022142
Produced by FastQC (version 0.11.5)