FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005088361

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005088361
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18012874
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTCAGTAACACTATCTTTAACTTTCTCACCTTCAGTTACACATTAAAT206990.11491225664488632No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCAG86150.036.6926271
GTAACAC89500.034.8250927
CTCAGTA90800.034.723363
TCTCAGT97350.032.0189482
TTAGACC40800.024.1706227
GTTAGAC43900.022.627976
TAACACT138800.022.5073958
TAGACCC44550.022.297518
CAGTAAC153800.020.6869725
TCAGTAA194950.016.4312344
TAGTTAG66700.015.8644464
AACACTA218250.014.5118719
AGTAACA247400.013.0931416
AGTTAGA83900.012.9552565
AGACCCC78500.012.6541939
TTAGCCC51250.012.645246120-124
TAGACTC53650.012.024639135-139
ATTAGCC68400.09.601019120-124
ACACTAG78250.08.41019770-74
GTTACAC86750.07.80548435-39
Produced by FastQC (version 0.11.5)