FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005088366

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005088366
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17621760
Sequences flagged as poor quality0
Sequence length150
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAG211210.11985749437059637No Hit
GAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGA176470.10014323200406769No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA231550.09.4217231
TCGGAAG247150.09.0018213
ATCGGAA278100.08.155342
AGAGCGT280650.07.59388
AAGAGCG316550.06.9827837
GGAAGAG349700.06.4855595
GAGCGTC355550.06.3383499
TAGATCT75200.06.340-44
GAAGAGC380150.06.0797056
CGGAAGA385600.05.78838164
TCATTAA90600.05.483443765-69
CTAGATC13600.05.294118140-144
AGTGTGA91800.05.03529425-29
Produced by FastQC (version 0.11.5)