FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005088415

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005088415
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16260068
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGAGT53300.018.7472521
TAACCCC53050.018.3217879
GTGAGTA56650.018.3142052
ATAACCC90250.011.168648
TGAGTAT130800.08.03640653
GAGTATA145050.07.59434754
GTATAAC149900.07.25174766
TATAACC161600.06.7275427
CGCCTAA46000.05.195905745-49
AGCATGA172150.05.14419659
AACCCCC46400.05.12033210-14
Produced by FastQC (version 0.11.5)