FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005088444

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005088444
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18321272
Sequences flagged as poor quality0
Sequence length150
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA205250.014.2395349
AGAGCAC238550.012.3121438
GATCGGA239650.012.1961731
TCGGAAG244500.012.0716813
ATCGGAA266650.010.9877562
AAGAGCA295150.010.5364117
CAATTCA74700.09.3475315
ACAATTC84550.09.2842074
GGCTAAT23200.08.7095272
GCTAATT24600.08.5071923
TAATTCA67550.07.99248275
GGAAGAG398950.07.7768665
GAAGAGC441300.07.3731036
TACAATT66950.07.3296233
CGGAAGA417850.07.30764444
AATTCAC79750.07.2211296
AGTCACG82150.07.151000525-29
GAGTCAA31101.8189894E-126.9961131
AATTCAG95000.06.9712396
GAATTCA113650.06.9040335
Produced by FastQC (version 0.11.5)