FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005088450

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005088450
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16266660
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGCTC55900.0119.0904161
CAGTACT107200.075.992363
AGTACTT126050.064.685114
TAGCTCA81200.061.9177062
GTACTTT172750.047.548565
GTTAAGC68000.044.2759784
GCGTTAA69250.043.16622
AGCTCAG121650.041.5649343
CGTTAAG75200.040.036913
TTAAGCT75700.039.4658975
TCAGTAC89500.038.550322
TAAGCTT82400.036.6063656
AGCGTTA87550.034.3825451
TTGCACC159100.033.655991
TACTTTT251000.032.9259226
TAGCTCT57750.029.1863212
ACTTTTT326850.025.6153937
GCTCAGC219450.023.631854
ACAGTAC83150.021.6567462
TGCACCA180600.019.782422
Produced by FastQC (version 0.11.5)