FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005088462

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005088462
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20324949
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTAAG38250.013.173996
GTCCCTA32950.012.2355013
CTAAGAG43450.010.7704568
CCTATAG41900.09.1056836
ACCCTAA59100.08.4045085
TACCCTA57300.08.1656194
CCTAAGA71900.07.1085367
CTATAGC61050.06.60317857
CCCTATA70600.06.4237195
TAAGAGC81000.05.95525469
GTACCCT92300.05.7719053
TCCCTAT86600.05.3197654
GCTAGTC9603.092282E-115.2505355130-134
TGTACCC102550.05.19500882
Produced by FastQC (version 0.11.5)