FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005088478

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005088478
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16315986
Sequences flagged as poor quality0
Sequence length150
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGCTC50650.0121.564071
CAGTACT110000.071.773553
AGTACTT132000.059.6468434
TAGCTCA76450.056.9128042
GCGTTAA49000.051.0133632
GTACTTT167100.047.0451135
GTTAAGC54000.046.4223064
CGTTAAG56250.044.8220863
TTAAGCT56300.044.625735
AGCTCAG107850.040.7433053
TAAGCTT64050.039.6756366
TTGCACC130250.038.2733651
TACTTTT230800.034.684656
TCAGTAC106300.031.6471182
ACTTTTT262200.030.6956947
TAGCTCT60000.030.3752482
AGCGTTA86250.029.6960931
GCTCAGC186850.023.979414
CTTTTTT409400.020.0986428
TGCACCA174200.018.5259482
Produced by FastQC (version 0.11.5)